![](https://parts.igem.org/images/partbypart/icon_translational_unit.png)
Translational_Unit
Part:BBa_K2230021:Design
Designed by: Yi-Lun Huang Group: iGEM17_Mingdao (2017-10-20)
RBS-ptsG/pSB1C3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal NgoMIV site found at 1224
Illegal AgeI site found at 508 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
No
Source
Cloning: The ptsG gene was amplified from gDNA of Salmonella typhimurium using a primer with RBS sequence (B0034), and then cloned onto pSB1C3